Development of a species-specific PCR-RFLP targeting rpoD gene fragment for discrimination of Aeromonas species
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چکیده
منابع مشابه
Development of species-specific PCR and PCR-restriction fragment length polymorphism assays for L.infantum/L.donovani discrimination.
Discrimination of Leishmania infantum and L. donovani, the members of the L. (L.) donovani complex, is important for diagnosis and epidemiological studies of visceral leishmaniasis (VL). We have developed two molecular tools including a restriction fragment length polymorphisms of amplified DNA (PCR-RFLP) and a PCR that are capable to discriminate L. donovani from L. infantum. Typing of the com...
متن کاملDevelopment of duplex-PCR for identification of Aeromonas species.
INTRODUCTION The number of reports of intestinal infections caused by Aeromonas spp. has increased significantly in recent years. In most clinical laboratories, identification of these bacteria is carried out by general phenotypic tests that sometimes do not accurately differentiate Aeromonas and Vibrio. METHODS A duplex-polymerase chain reaction (PCR) was developed directed to 2 targets iden...
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Background and Purpose: The epidemiological alteration in the distribution of Candida species, as well as the significantly increasing trend of either intrinsic or acquired resistance of some of these fungi highlights the need for a reliable method for the identification of the species. Polymerase chain reaction (PCR) is one of the methods facilitating the quick and precise identification of Ca...
متن کاملSpecies-specific identification of Campylobacters by PCR-restriction fragment length polymorphism and PCR targeting of the gyrase B gene.
PCR-restriction fragment length polymorphism (RFLP) analysis of a 960-bp fragment of the Campylobacter gyrB gene with either DdeI or XspI restriction enzymes generated unique digestion patterns for 12 different Campylobacter species. In addition, PCR assays using species-specific primer sets targeting gyrB were specific for the respective Campylobacter species. Therefore, PCR-RFLP analysis and ...
متن کاملIdentification of Avian Salmonella Isolates by PCR-RFLP Analysis of a fliC Gene Fragment
The genus of Salmonella is very polymorphic and comprised of a number of genetically closely related serotypes. It is one of the emerging pathogen in food-borne disease which is often found in contaminated chicken eggs. Salmonella enterica is considered one of the major pathogens in public health worldwide. A total of 31 Salmonella isolates identified by specific antisera, which included Salmon...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2018
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.000796